Dark field microscope. Definition. How to use

In this article we will consider the following points:

- How the dark field microscope.
- What to look for this type of microscope.
- Their application to the field of biology, medicine and health education.
- Definition of dark field microscopy (darkfield blood).



How the dark field microscope

Because of the angle of incidence of the beam on the specimen being observed, allowing the background is dark and the edges of the blood cells appear bright. This is possible due to the type of capacitor that has this type of microscope. The light is scattered when hitting the cell or specimen being observed. It's like entering a dark room in a small beam of light through a crack in a window and into the dark room illuminated dust particles floating in the air. What was initially invisible becomes visible. To understand how the dark-field microscopy we have a video that makes us view this type of technique.

What to watch this kind of microscopes
- Particles dispersed in a homogeneous medium.
- Observation of Brownian motion of particles.
- Can be used for observation of living preparations, not colored.
- Visualize the leading edges of the samples.
- Y microorganisms with diameters greater than 0.2 um.

RBC darkfield
So with this type of microscopy colloidal particles can be seen from the blood.

Its application to the field of biology, medicine and health education
Of course this technique is used in various scientific fields. However, it was Prof. Dr. Günther Enderlein who with his research on blood cells and plasma has been popular in the field of biological medicine mainly in Europe and more specifically in Germany.

It is in this country which has published major books that speak of this technique aimed at emphasizing or guidelines that left Enderlein and pleomorphism.
Some of these are: "Ciclogenia bacteria (Bacteria Cyclogeny) Günther Enderlein.

In its most fervent advocates have Mary M. Bleker has published "Blood test in dark field (im Blutuntersuchung Dunkelfelder) and the most recent published in 2004" anonymous friend "the enemy or stranger?" (Verkannte Der Freund oder der unbekannte Feind).

Another book is "Introduction to Field Diagnostics Dark" (Einführung in die Dunkelfelddiagnostik) by Cornelia Franz Schwerdtle and Arnoul ..

All this has been the result of the struggle to defend each other as irreconcilable positions has been the monomorphism and polymorphism.

So from the beginning of last century until now has been used to study the blood cells and plasma in vivo and without stains.

It draws a drop of blood from a finger (we'll discuss how to extract this type) is placed on a slide and covered with a coverslip. Immersion oil is placed on the condenser, dark field microscope after the sample on the microscope stage. Below is the capacitor rises until it contacts the carrier and disperse the oil on the sample below the glass. Then he begins to focus with the aim of increasing and there is less blood in dark field. Increases to about 500 we can see without using more oil. However, to observe all the information that we can give blood you must use the objective 1000X. For this, again take a drop of immersion oil on top of the coverslip and place the objective of 1000X on the sample until they make contact just to see blood cells and colloids in all its glory.

The two stages of contact with the sample, the condenser below the target of 100 and above are most sensitive to have excellent vision.

Definition of dark field microscopy for health

We could define the blood in dark field microscopy as the technique to study in vivo, without stains or substances added to human blood to better understand the health of the individual deficiencies and excesses associated with diet, anemia, vitamins, toxic waste, and pathological predispositions especially under endobiosis pleomorphism.

More about using the dark field microscope:
* Dark-field microscope. Research sistatogenia
* Dark-field microscope. Pleomorphism and monomorphism
* Dark-field microscope: Günther Enderlein and Maria M. Bleker

*Automatic Translation